Influenza virus hemagglutinin and neuraminidase cytoplasmic tails control particle shape

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Influenza virus hemagglutinin and neuraminidase cytoplasmic tails control particle shape.

The cytoplasmic tails of the influenza virus glycoproteins hemagglutinin (HA) and neuraminidase (NA) are highly conserved in sequence for all virus subtypes and it is believed that assembly of this enveloped virus depends on interactions of these domains with cytoplasmic viral components. However, it is possible to rescue altered influenza viruses lacking either the HA or NA cytoplasmic tails. ...

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Influenza virus neuraminidase.

Antiserum specific for influenza A2 neuraminidase was produced by immunization of rabbits with the purified enzyme which had been isolated by electrophoresis from the proteins of a detergent-disrupted AoA2 influenza virus recombinant [X-7 (Fl)]. This recombinant contained hemagglutinin of the Ao subtype and A2 neuraminidase. Antiserum to the isolated A2 neuraminidase did not react in any of fou...

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Label‐free mass spectrometry‐based quantification of hemagglutinin and neuraminidase in influenza virus preparations and vaccines

BACKGROUND Influenza vaccination is the primary method for preventing influenza and its severe complications. An accurate rapid method to determine hemagglutinin (HA) concentration would facilitate reference antigen preparation and consequently expedite availability of seasonal as well as pandemic vaccines. OBJECTIVE The goal of this study was to develop a label-free mass spectrometry (MS) ba...

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The Interplay between the Host Receptor and Influenza Virus Hemagglutinin and Neuraminidase

The hemagglutinin (HA) and neuraminidase (NA) glycoproteins of influenza A virus are responsible for the surface interactions of the virion with the host. Entry of the virus is mediated by functions of the HA: binding to cellular receptors and facilitating fusion of the virion membrane with the endosomal membrane. The HA structure contains receptor binding sites in the globular membrane distal ...

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Balanced hemagglutinin and neuraminidase activities are critical for efficient replication of influenza A virus.

The SD0 mutant of influenza virus A/WSN/33 (WSN), characterized by a 24-amino-acid deletion in the neuraminidase (NA) stalk, does not grow in embryonated chicken eggs because of defective NA function. Continuous passage of SD0 in eggs yielded 10 independent clones that replicated efficiently. Characterization of these egg-adapted viruses showed that five of the viruses contained insertions in t...

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ژورنال

عنوان ژورنال: The EMBO Journal

سال: 1997

ISSN: 1460-2075

DOI: 10.1093/emboj/16.6.1236